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Registros recuperados: 7
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Anil-Babesia rodhaini Monoclonal Antibodies: Effect against Babesia rodhaini and Cross-reactivity with Human Starin of Babesia microti OAK
Claveria, Florencia G.; Omata, Yoshitaka; Takehara, Tokuma; Saito, Atsushi; Maki, Yoshiyuki; Igarashi, Ikuo; Suzuki, Naoyoshi.
Anti-Babesia rodhaini monoclonal antibodies (mAb), namely: 1-E7, 2-H2 and 3-B8, significantly suppressed the development of high parasitemia in BALB/c mice infected with B. rodhaini and all mAb-treated mice survived the infection. While, only monoclonal antibody 3-B8 showed some inhibitory effect against Babesia microti, with mice showing high parasitemia of 18.04 ± 2.69 %, at 9 days post-exposure. Westernblot analysis of B. rodhaini and B. microti parasite extract reacted with anti-B. rodhaini monoclonal antibodies showed cross-reactive bands of molecular weights 62 and 55 kilodaltons. Comparison of the antigenic components of B. rodhaini and B. microti using polyspecific sera revealed several shared or common parasite antigens of molecular weights 62,...
Palavras-chave: Monoclonal antibodies; Cross-reactivity; Babesia rodhaini; Babesia microti.
Ano: 1992 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/171
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Cellualr Subsets Involved in Protective Immunity to Babesiarodhaini Infection in BALB/c Mice OAK
Shimada, Terumasa; Igarashi, Ikuo; Maki, Yoshiyuki; Claveria, Florencia G.; Saito, Atsushi; Suzuki, Naoyoshi.
Protective immune responses and the functional role of spleen cells in mice infected with Babesia rodhaini were examined with an in vitro proliferation assay systems and by in vivo passive transfer of spleen cells to uninfected mice. Mice that resolved primary babesial infection after chemotherapy (Babesia immune mice) had transient and low parasitemia after challenge infection and high rates (75%) of survival. Babesia hyperimmune mice, by contrast, had no detectable parasitemia after challenge and 100% survival. Proliferative response of spleen cells to Babesia lysate antigen (BLA) were determined for mice from both groups. This proliferative response was inhibited by treatment of spleen cells with anti-T cell serum and monoclonal antibody (MAb) to Lyt l...
Palavras-chave: Babesia rodhaini; Cell transfer; Spleen cells; Protective immunity.
Ano: 1991 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/157
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Non-invasive Method of Identification of SAG-1 Transgenic Mice by PCR Analysis of Oral Wash Cells OAK
Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Suzuki, R.; Kato, Mihoko; Bray, R. L.; Lim, C.; Zayatiin, Batsukh; Kamada, Takenori; Inoue, Noboru; Xuan, Xuenan; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Mikami, Takeshi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 玄, 学南.
Palavras-chave: SAG-1; Oral wash and two-step polymerase chain reaction.
Ano: 1999 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/311
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Production of Transgenic Mice Carrying p30 Gene Encoding Major Surface Antigen of Toxoplasma gondii OAK
Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Inoue, Noboru; Igarashi, Ikuo; Nagasawa, Hideyuki; Horiuchi, Motohiro; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 五十嵐, 郁男; 長澤, 秀行.
Palavras-chave: Toxoplasma gondii; SAG-1 (p30); Transgenic mice.
Ano: 1996 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/254
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Restorative Effects of A Newly Synthesized Peptide, Obiopeptide-1, in Cyclophosphamide- or Carrageenan-pretreated Mice Infected with Opportunistic Bacteria OAK
Fujii, Yasuhiro; Maki, Yoshiyuki; Ito, Masanari; Saito, Atsushi; Igarashi, Ikuo; Ono, Kenichiro; Itoh, Kikuji; Suzuki, Naoyoshi.
Large numbers of cyclophosphamide- or carrageenan-pretreated immunosuppressed adult ddY mice died within 10 days after inoculation with several species of opportunistic bacteria, including P. aeruginosa, K. pneumoniae, E. coli, S. aureus and methicillin resistant S. aureus. When immunosuppressed mice were administered bacteria in combination with two 100 μg/mouse intramuscular doses of a newly synthesized peptide, Obiopeptide-1 (OP-1), survival rates increased significantly. At 24 and 48 hours after intraperitoneal inoculation with P. aeruginosa, counts of viable organisms from the livers, spleens, lungs, hearts, and kidneys of mice that were administered cyclophosphamide in combination with OP-1 were significantly lower than counts from mice that were...
Palavras-chave: Immunoregulator; Synthesized peptide; Immunosuppression; Cyclophosphamide; Carrageenan; Ooiopeptide.
Ano: 1992 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/166
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Sequence Analysis of Three Major Antigens (P30, P23 and P22) of Virulent and Avirulent Strains of Toxoplasma gondii OAK
Maki, Yoshiyuki; Seng, Seyha; Kato, Mihoko; Hoshi, Yukihiro; Igarashi, Ikuo; Nagasawa, Hideyuki; Toyoda, Yutaka; Suzuki, Naoyoshi.
Palavras-chave: Toxoplasma gondii; SAG-1 (P30); CDNA.
Ano: 1996 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/250
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Toxoplasma Lysate Antigen (TLA144)-Ig8 Antigenic Component : Binding and Induction of Cytotoxic Cell Activity in Mouse Spleen Cells OAK
Kato, Mihoko; Maki, Yoshiyuki; Inoue, Noboru; Omata, Yoshitaka; Claveria, Florencia G.; Igarashi, Ikuo; Saito, Atsushi; Suzuki, Naoyoshi; 井上, 昇; 五十嵐, 郁男.
Palavras-chave: Cytotoxic cell activity; NK cell; LAK cell; Toxoplasma lysate antigen.
Ano: 1994 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/216
Registros recuperados: 7
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